Total lipids were determined gravimetrically, and sterols analysed by GC/MSD. Date: 02 nd August, 2014. agitated at 150 rev/min for 13 days. RSJ1 and its application in ester hydrolysis, Optimization of Lipase Production by Pseudomonas spp. Pingkan Aditiawati. carried out using tributyrin agar plates (0.5%, described by Lawrence (1967) with slight, colonies, indicative of lipase activity. 1997; 59: 37. processes Journal of Industrial Microbiology and Biotechnology. C20 [8]. Stock cultures were carried on tryptone-glucose -beef extract agar slants which were stored at 2 to 3 ° C. and were transferred at monthly intervals. analysis was carried out using universal primer 27F and 1492R. In: Tourbier J, Pierson RW, Biological Control of Water Pollution, The presence of Fats, Oils and Greases (FOGs) in restaurant waste water is a major problem. However, there was a lag phase of about 1 day in every case. Structure of lipase from Pseudomonas aeruginosa (Source: Jaeger and Reetz, 1998). The absence of a lag phase resulted in faster growth and fat removal. (L5). Growth of the organisms and lipase production were measured with varying pH (4 - 9) temperature (27, 37 and 47ºC) and various substrate concentrations. B. Naik Arts and Science College,India, Chikhali. Prokaryotic abundance (PA) was determined by epifluorescence microscopy (DAPI staining), and faecal indicator bacteria (FIB) enumerated with the multiple test tube and most probable number method. The fate of organic oil and fat (i.e. The hydrolyzed free form of glycidol has been categorized as probably carcinogenic to humans by the World Health Organization’s International Agency for Research on Cancer. Factors Affecting Lipase Production, Lipases: Interfacial Enzymes with Attractive Applications, The realm of microbial lipases in biotechnology, Development of economical mosquito repellent using marigold plant, Comparative Nutritional analysis of few accessions of Nigerian & Indian Eggplants, Isolation and Characterization of Lipase Producing Bacteria from Windrow Compost, Isolation And Characterization Of Lipase Producing Bacteria From Restaurant Waste Water. in submerged batch process in shake flask culture, Thermostable alkaline lipase from a newly isolated thermophilic Bacillus, strain A30-1 (ATCC 53841), Production of exogenous lipases by bacteria, fungi, and actinomycetes, The Lipase of Pseudomonas fragi1 II. The optimum pH for A2, B4, C1 and C2, enzymes and are known to bring about a, changes in appearance as a result of lipolysis, microbial colonies on solid media depending, producing microorganisms. Dynamics of prices of derived oil crop products in the international market is conditioned by the relationship, Mineral oil hydrocarbon contaminations can often be found in oilseeds, oils, and fats as these matrices are good keepers to accumulate them from all kind of sources along the production chain. The pure cultures were Acinetobacter sp., Rhodococcus rubra, Nocardia amarae and Microthrix parvicella and these were compared with a mixed culture isolated from a greasetrap, MC1, and with activated sludge. As the result, most commercially produced lipases have been produced from fungi and bacteria. The optimum conditions for lipase production of Pseudomonas aeruginosa HBUZL-6 showed that the maximum production of lipase occurs during 48 hrs of incubation of fermentation medium and at temperature 35 °C, pH 6 and 150 rpm of agitation speed in presence 1 % of olive oil as substrate and carbon source for reaching the maximum lipase activity about 229 U/ml. Isolation and Optimization of Lipase Producing Bacteria. There are many approaches for designing an ideal fermentation process. The lipase test substrate and the determination of lipase activity were the same as outlined previously (6). Mechanism of Lipase action (Source: Jaeger and Reetz, 1998). In a controlled fermenter (1 L), the lipase activity was found to increase by 36% (12 Uml(-1)). The traditional approach to the optimization problem is the one variable at a time method. Shows the hydrolytic activity of lipase producer (isolate B4) on TBA plate. and P. diminuta) produced the highest degradative activity, followed by M3 (Pseudomonas sp., P. diminuta and P. pseudoalcaligenes). RSJ1 from hot-spring, grew in temperature range of 40 - 65°C and a pH range of 5 - 12 with the optima at 50°C and pH 9. observed visually, every 24 hrs for growth. Dr. Patangrao Kadam Mahavidyalaya, Sangli. Microbial lipases have been exploited in the food and detergent industries (Pandey et al, 1999; Saxena et al, 1999), chemical and pharmaceutical industry owing to their activity in micro-aqueous media (Jaeger and Reetz, 1998). Unusually versatile substrate specificity is shown by lipases. INNSPUBs Speech; Aim and Activities; Representative Offices; Contact Us; Journals . État : Neuf . The lipase production was maximum at pH7, temperature 370C and incubation time 48 hours by the lipase producing bacteria BLP2 Pseudomonas gessardii. The half life of the enzyme was 8 h at 75°C. Hungarian Journal of Industrial Chemistry. The book … Journal List; J Insect Sci; v.11; 2011; PMC3391909; J Insect Sci. Akhmaloka Akhmaloka . The effects of fish cannery discharge (FCD) on bacteria in marine coastal sediments were investigated. Before in- oculating test media, the cultures were transferred twice in broth at 21 ° C. for 24 hours; one drop of the second transfer was inoculated into the growth medium, which was incubated at 15 ° C. for 3 days unless otherwise stated. This is of special importance for the food survey and for the production as it is a challenging task to survey the many routes of contamination along the production chain and during storage in order to improve the food quality. The … Lipase-producing bacteria that are found in the rumen are responsible for freeing unsaturated fatty acids from a glycerol backbone, thereby allowing the double bond to be saturated. Lipase activity and production depend upon the composition of the fermentation medium (Kim et al, 1996). yeast, fungi and bacteria, for example, Candida rugosa [1] Thermomyces lanuginosus [2] Fusarium oxysporum f. sp. There are numerous varieties of oils which can release their energy on burning. http://dx.doi.org/10.20546/ijcmas.2016.508.023. List of potential lipase producing bacteria. considered as indicative positive result. isolates were checked for their potential to secrete enzyme Lipase. Lipases are being, products. Various methods of lipase assay have been classified as; Titrimetry, Interfacial tensiometry, Spectroscopy, Chromatography, Immunochemistry and Conductimetry (Frédéric et al, 2000; Kulkarni, 2002) of these methods titrimetry is the simplest method but needs continuous readout by pH meter; the so called pH stat method. All sediments were anoxic with extremely negative potentials around the outfall. Join ResearchGate to find the people and research you need to help your work. Enrichment and Isolation of lipolytic bacteria, comparing with control. The growth kinetics of the commercial and native inocula followed Haldane and Monod models respectively. Overall fat removal values were above 78% for both inocula. Breuil C, Shindler DB, Sijher JS, Kushner DJ. for 15 minutes. PDF | On Aug 15, 2016, Miral Patel and others published Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site | Find, read … Efficacy evaluation, molecular and biochemical characterization of lipase producing bacterial isolates. … Bioresour Technol. The isolates were identified using morphological, physiological and biochemical tests. Palm oil induces the least Lipase production, (less than 6 Units/ ml, ground nut oil (Figure 4). You might not require … for vegetable fermentations. Their amount, for example, in municipal wastewater is approximately 30-40% of the total chemical oxygen demand. Join ResearchGate to find the people and research you need to help your work. the accession numbers. Fish cannery discharges can also contain specific bacterial groups able to produce extracellular lipase enzymes that hydrolyze triglycerides and cholesterol [3][4][5][6][7], A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content. (Henry et al, 1997). The aim of this research was to study the formation of glycidyl esters during the. None of the metal ion salts, other than calcium chloride and sodium chloride, incorporated in the production medium improved the lipase yield. Putu Parwata. Redox potentials were measured, and granulometry was determined by wet ASTM sieving, and with the Sedigraph method. lab-scale deodorization of the three most widely produced seed oils in the world (sunflower, rapeseed and soybean). Optimal lipase activity was achieved in medium using combination of sunflower oil and Tween 80 (1% v/v each) as carbon sources. One of the twenty isolated strain exhibited a greater zone of clearance than the others indicating higher lipase activity was selected and identified based on their, A superior lipase-producing bacterium was isolated from forest soil samples in Setapak, Malaysia and it was identified as Burkholderia cenocepacia with 16S rRNA sequencing technique. The enzyme activity was stable to both hydrogen peroxide and alkaline protease which are detergent ingredients. Isolation and Identification of Lipase-producing Bacteria from Oil-Contaminated Soils Khaing Phyo Wai1,Weine Nway Nway Oo2 1Department of Biotechnology, Mandalay TechnologicalUniversity, MandalayRegion, Republic of the Union of Myanmar 2Department o f Biotechnology, TechnologicalUniversity(Kyaukse) ,KyaukseRepublic of the Union of Myanmar … Boonmahome P, Mongkolthanaruk W (2013) Lipase-producing bacterium and its enzyme characterization. However, fuels derived from triglycerides (vegetable oils/animal, Glycidyl esters are foodborne contaminants formed during the production of fats and oils, especially during the deodorization of palm oil. A thermophilic isolate, Bacillus sp. Isolation and characterization of Lipase producing Bacteria from vegetable oil spillage site, Indo-Asian Journal of Multidisciplinary Research (IAJMR) STUDY ON THE ACTIVITY AND OPTIMIZATION OF LIPASE PRODUCING BACTERIA ISOLATED FROM OIL CONTAMINATED SOIL, Screening and production of lipase producing bacteria from vegetable oil industry, Thermotolerant strain of Bacillus licheniformis producing lipase, Review of methods of enumeration, detection and isolation of lipolytic microorganisms with special reference to dairy applications, Production of extracellular alkaline lipase from a Bacillus sp. Since last few decades, continuous efforts are being made to screen the lipase producing bacterial strains from the environment because of its diverse comm. [PMC free article] Goldsack DE. The purified enzyme had an isoelectric point of 5.15 and an approximate molecular weight of 65,000. Sneath, HA, Mair, NS, Sharpe ME and Holt JG (Eds). J Life Sci Technol 1(4):196–200 Google Scholar Ghasemi Y, Rasoul Amini S, Kazemi A, Zarrinic G, Morowvat MH, Kargar M (2011) Isolation and characterization of some moderately halophilic bacteria with lipase activity. The substitution of animal fats with vegetable oils in human nutrition, the expansion of biodiesel industry and intensification of livestock production have led to increased demand for oil crops. Isolation, Characterization of Lipase Producing Bacteria from Crude Rice Bran Oil and Optimization studies by Response Surface Methodology (RSM) Lipases are enzymes that can be secreted by several …